<?xml version="1.0" encoding="ISO-8859-1"?><article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance">
<front>
<journal-meta>
<journal-id>1688-4809</journal-id>
<journal-title><![CDATA[Veterinaria (Montevideo)]]></journal-title>
<abbrev-journal-title><![CDATA[Veterinaria (Montev.)]]></abbrev-journal-title>
<issn>1688-4809</issn>
<publisher>
<publisher-name><![CDATA[Sociedad de Medicina Veterinaria del Uruguay]]></publisher-name>
</publisher>
</journal-meta>
<article-meta>
<article-id>S1688-48092021000201202</article-id>
<article-id pub-id-type="doi">10.29155/vet.57.216.3</article-id>
<title-group>
<article-title xml:lang="es"><![CDATA[Diagnóstico molecular de Neospora caninum en fetos abortados espontáneamente en bovinos de Uruguay]]></article-title>
<article-title xml:lang="en"><![CDATA[Molecular diagnosis of Neospora caninum in aborted bovine fetus from Uruguay]]></article-title>
</title-group>
<contrib-group>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Briano]]></surname>
<given-names><![CDATA[Carolina]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Regidor-Cerrillo]]></surname>
<given-names><![CDATA[Javier]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Easton]]></surname>
<given-names><![CDATA[Cristina]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Pieruccioni]]></surname>
<given-names><![CDATA[Florencia]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Romero]]></surname>
<given-names><![CDATA[Agustín]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Peraza]]></surname>
<given-names><![CDATA[Pablo]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Mederos]]></surname>
<given-names><![CDATA[América]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
<contrib contrib-type="author">
<name>
<surname><![CDATA[Dutra]]></surname>
<given-names><![CDATA[Fernando]]></given-names>
</name>
<xref ref-type="aff" rid="Aff"/>
</contrib>
</contrib-group>
<aff id="Af1">
<institution><![CDATA[,M.G.A.P División de Laboratorios Veterinarios "Miguel C. Rubino" (DI.LA.VE) ]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>Uruguay</country>
</aff>
<aff id="Af2">
<institution><![CDATA[,Instituto Nacional de Investigación Agropecuaria (I.N.I.A.) Facultad de Veterinaria, Universidad Complutense de Madrid Grupo SALUVET. Departamento de Sanidad Animal]]></institution>
<addr-line><![CDATA[ ]]></addr-line>
<country>España</country>
</aff>
<aff id="Af3">
<institution><![CDATA[,Estación Experimental I.N.I.A Unidad de Biotecnología ]]></institution>
<addr-line><![CDATA[Las Brujas, Ruta 48 Km10, Canelones ]]></addr-line>
<country>Uruguay</country>
</aff>
<aff id="Af4">
<institution><![CDATA[,Programa Producción de Carne y Lana. Estación Experimental I.N.I.A. Tacuarembó Instituto Nacional de Investigación Agropecuaria (I.N.I.A.) ]]></institution>
<addr-line><![CDATA[Ruta 5 km 386, Tacuarembó ]]></addr-line>
<country>Uruguay</country>
</aff>
<pub-date pub-type="pub">
<day>00</day>
<month>12</month>
<year>2021</year>
</pub-date>
<pub-date pub-type="epub">
<day>00</day>
<month>12</month>
<year>2021</year>
</pub-date>
<volume>57</volume>
<numero>216</numero>
<copyright-statement/>
<copyright-year/>
<self-uri xlink:href="http://www.scielo.edu.uy/scielo.php?script=sci_arttext&amp;pid=S1688-48092021000201202&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.edu.uy/scielo.php?script=sci_abstract&amp;pid=S1688-48092021000201202&amp;lng=en&amp;nrm=iso"></self-uri><self-uri xlink:href="http://www.scielo.edu.uy/scielo.php?script=sci_pdf&amp;pid=S1688-48092021000201202&amp;lng=en&amp;nrm=iso"></self-uri><abstract abstract-type="short" xml:lang="es"><p><![CDATA[Resumen: En Uruguay el diagnóstico de rutina de Neospora caninum se realiza fundamentalmente por estudios histopatológicos de los fetos abortados, complementando esta información con resultados de serología y/o Inmunohistoquímica (IHQ). El objetivo de este estudio fue estandarizar un protocolo de amplificación de secuencias mediante PCR (polymerase chain reaction) para la detección de Neospora caninum en órganos de fetos abortados espontáneamente en bovinos para su incorporación a la rutina diagnóstica. Con este propósito se realizó un estudio retrospectivo utilizando muestras congeladas de archivo, de un periodo comprendido entre los años 2013 y 2016, con diagnóstico positivo o negativo a Neospora caninum. Los resultados mostraron que de 31 muestras positivas a Neospora caninum por las técnicas de rutina, 26 fueron positivas por PCR (84%), demostrando una alta sensibilidad de diagnóstico de esta técnica. Todas las muestras con diagnóstico negativo mediante histopatología (n=20) fueron confirmadas como negativo mediante PCR. La comparación de los resultados de PCR con los de histopatología, mediante el test de Cohen&#8217;s Kappa, señalaron un acuerdo muy alto (81%, p&lt;0.001) entre ambos diagnósticos. Dichos resultados sumados a la simplicidad y rapidez de la técnica podrían sugerir a la PCR como alternativo de la IHQ como técnica confirmatoria en el diagnóstico de rutina. También permitiría realizar análisis retrospectivos de muestras archivadas, paso fundamental para futuros estudios genéticos de la población de Neospora caninum presente en Uruguay.]]></p></abstract>
<abstract abstract-type="short" xml:lang="en"><p><![CDATA[Abstract: The diagnosis of Neospora caninum in Uruguay is carried out by histopathological studies of aborted fetuses, complementing this information with serology test and/or immunohistochemistry (IHC) results. The aim of this study was to standardize a protocol of polymerase chain reaction (PCR) to detect of N. caninum in organs of spontaneously aborted bovine fetus. A retrospective study was conducted using frozen samples from a period between 2013 and 2016, with positive or negative diagnosis of N. caninum. Results showed that 26 of 31 samples positives for N. caninum by routine techniques were positive by PCR, demonstrating a high sensitivity of the PCR. All negative samples by Histopathology (n=20) were confirmed as negative by PCR. Cohen's Kappa test indicated a very high agreement (81%, p&lt;0.001) between PCR and Histopathology with serology test and/or immunohistochemistry (IHC) results. The high sensitivity, specificity and simplicity of PCR test suggest this technique as an alternative to IHC as a confirmatory technique in routine diagnosis.]]></p></abstract>
<kwd-group>
<kwd lng="es"><![CDATA[Diagnóstico]]></kwd>
<kwd lng="es"><![CDATA[Neospora caninum]]></kwd>
<kwd lng="es"><![CDATA[PCR]]></kwd>
<kwd lng="en"><![CDATA[Diagnosis]]></kwd>
<kwd lng="en"><![CDATA[Neospora caninum]]></kwd>
<kwd lng="en"><![CDATA[PCR]]></kwd>
</kwd-group>
</article-meta>
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