SciELO - Scientific Electronic Library Online

vol.16 issue1Relationship Among Precipitation, Temperature, Lemon (Citrus limon) Leaf Susceptibility and the Appearance of CankerEvaluation of Methods to Disinfect Tomato Seeds Against Bacterial Canker (Clavibacter michiganensis subsp. michiganensis) author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand



Related links


Agrociencia (Uruguay)

Print version ISSN 1510-0839On-line version ISSN 2301-1548


PEYROU, Mercedes et al. Biological, Serological and Molecular Techniques to Xanthomonas Axonopodis pv. Citri Asymptomatic Detection. Agrociencia Uruguay [online]. 2012, vol.16, n.1, pp.124-133. ISSN 1510-0839.

Xanthomonas axonopodis pv. citri (Xac) produces citrus canker disease in all citrus commercial species. The bacteria can be disseminated through vegetative propagation material in asymptomatic form. To optimize bacteria detection techniques applicable to asymptomatic citrus plant tissue routine analysis, ELISA, Immunofluorescence, PCR, qRT_PCR and host plant inoculation (bioassay) diagnostic techniques were compared. Tests were made from decimal dilutions between 108 ufc.mL-1 and 102 ufc.mL-1 using a pure culture of 49b strain.The detection level obtained was 1.8 x 102 ufc.mL-1 using Inmunofluorescence; 1.8x104 ufc.mL-1 with indirect ELISA, 1.8 x 103 ufc.mL-1 by means of PCR; 10 ufc.mL-1 through of qRT_PCR and 230 ufc.mL-1 in sour orange inoculated plants. The experiment was repeated at least three times for each technique. Considering this result, and taking into account that sensitivity, practicity and cost, are important when a great number of plants need to be tested, the PCR and inoculation in host plants were those that met the best characteristics to be evaluated in asymptomatic plant material


        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License