SciELO - Scientific Electronic Library Online

vol.15 issue2Arbuscular Mycorrhizas in Two Native Grasses of Uruguay in Grazed and Ungrazed AreasModels for Quantifying Winter Effective Chilling Adapted to Uruguayan Apple Production author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand



Related links


Agrociencia (Uruguay)

Print version ISSN 1510-0839On-line version ISSN 2301-1548


GALLINO, J. Pablo; VIDAL, Sabina; WELIN, Björn  and  PAGLIANO, Gabriela. Performing Gene Constructs for the Silencing of Tristeza and Psorosis Viruses in Citrus. Agrociencia Uruguay [online]. 2011, vol.15, n.2, pp.11-18. ISSN 1510-0839.

The citrus sector is the most important horticultural crop in Uruguay, accounting for the 95% of the fruit exports and being the third agricultural product in order of importance. Citrus Tristeza virus (CTV) and Citrus Psorosis virus (CPsV), together with the viroid for Exocortis and other viroids associated with this disease, are the viral and viroidal agents of highest incidence on citrus crops in our country. A highly effective strategy to control viral diseases is the induction of resistance by means of methods based on RNA silencing. RNA silencing is a mechanism based on the specific degradation of RNA, which is triggered by the presence of double-stranded RNA inside the cells. Recently, strategies to effectively silence any chosen RNA sequence have been described. Using constructs which are inserted into the plant genome and promote the formation of double-stranded RNA, it is possible to silence the expression of both endogenous genes and viral gene sequences. In this study, partial sequences of CTV and CPsV capsids were isolated and used to produce gene contructs that promote the formation of double-stranded RNA. These constructs consist on the insertion of sense and antisense viral sequences, separated by intron. The resulting constructs will be used in the genetic transformation of citrus in order to obtain plants resistant to these pathogens.


        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )


Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License