Abstract

ROMANELLI, Gerardo et al. In Vitro Human Megacaryocytopoiesis: Determination of Thrombopoietin Optimal Concentration. Anfamed [online]. 2019, vol.6, n.2, pp.25-34.  Epub Dec 01, 2019. ISSN 2301-1254.  https://doi.org/10.25184/anfamed2019v6n2a1.

The study of human megakaryocytopoiesis has been hampered by the relative scarcity of megakaryocytes in bone marrow (0.05-0.2 % of medullary cells), which has led to the optimization of protocols of in vitro expansion of precursors from diverse sources (umbilical cord, bone marrow and peripheral blood with or without previous mobilization). Cell cultures from different precursors have allowed the production and study of megakaryocytes as well as proplatelets and platelets. However, the in vitro production of megakaryocytes that culminate all stages of differentiation is a challenge that has not yet been resolved. In this work we report the findings related to the determination of thrombopoietin treatment conditions and concentrations to achieve an optimal relationship between the amount of thrombopoietin and the percentage and degree of megakaryocytic differentiation in five allogeneic donors that were accepted for bone marrow transplantation.

Keywords : Megakaryocytopoiesis; Thombopoietin; Hematopoietic Precursors; Allogeneic Donors; Cell Differentiation.

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