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Odontoestomatología
versão impressa ISSN 0797-0374versão On-line ISSN 1688-9339
Resumo
D´AIUTO, Natali et al. Study of the effects of a hypoxic microenvironment on human keratinocytes in vitro and its correlation with microenvironmental alterations in oral lichen planus pathology. Odontoestomatología [online]. 2022, vol.24, n.40, e223. Epub 01-Dez-2022. ISSN 0797-0374. https://doi.org/10.22592/ode2022n40e223.
Hypoxia is a fundamental factor in the process of tumor genesis, as well as in precursor pathologies of cancer, such as Oral Lichen Planus (OLP).
Objective:
To determine if it is possible to establish a correlation between the alterations that normal keratinocytes suffer in a hypoxic microenvironment in vitro and alterations that appear in the keratinocytes in the epithelium of the oral mucosa in the context of OLP pathology.
Methods:
Morphological changes were studied by phase contrast microscopy, and the detection of markers associated with hypoxia of human keratinocytes (HaCaT), as an oral cell model, in a hypoxic microenvironment generated by the variant of the method “Hypoxia induced by coverslips”.
Results:
Using confocal microscopy, the presence of hypoxia markers GLUT-1 and Hipoxyprobe was observed in HaCaT cell cultures exposed to a hypoxic microenvironment. In addition, the presence of the GLUT-1 marker was observed by immunohistochemistry in human epithelial tissue derived from biopsies of OLP pathology.
Conclusions:
A correlation was established between the alterations detected in human keratinocytes induced in a hypoxic microenvironment in vitro and the alterations detected in vivo in epithelial tissue of the oral mucosa.
Palavras-chave : Hypoxia; Oral Lichen Planus; cancer; keratinocytes.
