Resumen

DIANA, Leticia; CIUFFO, Camila  y  MUSTO, Héctor. Identification and characterization of methicillin-resistant Staphylococcus isolated from dogs. Veterinaria (Montev.) [online]. 2019, vol.55, n.212, pp.45-51.  Epub 01-Dic-2019. ISSN 0376-4362.  https://doi.org/10.29155/vet.55.212.1.

Staphylococcus aureusandStaphylococcus pseudintermediusare important pathogens in both humans and animals mainly due to its high prevalence and their widespread antibiotic resistance. Other species belonging to this genus could play a fundamental role in the expansion of resistance genes, acting as carriers and disseminators; thus, this genus has a major impact on veterinary medicine. The aim of this work was to detect and characterize strains ofStaphylococcus spp. methicillin-resistant isolated from dogs of Montevideo, including healthy dogs (carriers) and diseased dogs (with ears and skin infections). Samples were collected from animals that where admitted to the Hospital Center of the School of Veterinary Medicine - Universidad de la República during a one year period. Identification at the species level was carried out through 16S rRNA gene sequencing and the resistance profiles were determined by antibiogram. Sixty seven isolates belonging to nine species were identified: S. pseudintermedius, S. argenteus, S. cohnii, S. haemolyticus, S. epidermidis, S. arlettae, S. scheiflieri, S. xylosus and S. nepalensis. Seven strains were resistant to methicillin, and all of these were found to carry the mecA gene. Ten other strains were classified as MDR (multi drug resistance) since phenotypic studies indicated resistance to more than three classes of antibiotics. We conclude that the conditions for the transfer of methicillin resistance genes in canines are given by the existence of genes and the mobile genetic elements in which they are transported, as well as the presence of bacterial strains able to incorporate them.

Palabras clave : Staphylococcus aureus; Staphylococcus pseudintermedius Multi drug resistence; Dogs.

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