SciELO - Scientific Electronic Library Online

 
vol.32 número3Capacidad resolutiva del primer nivel de atención: experiencia de la Unidad Docente-Asistencial de Medicina Familiar y Comunitaria de Paysandú (2014)Evaluación del estado nutricional de yodo en una población de embarazadas índice de autoresíndice de materiabúsqueda de artículos
Home Pagelista alfabética de revistas  

Servicios Personalizados

Revista

Articulo

Indicadores

Links relacionados

  • No hay articulos similaresSimilares en SciELO

Compartir


Revista Médica del Uruguay

versión impresa ISSN 0303-3295versión On-line ISSN 1688-0390

Resumen

GONZALEZ, Evangelina et al. Estudio del ratio de FLT3-ITD como factor pronóstico en leucemias agudas mieloides: primeros casos estudiados en Uruguay. Rev. Méd. Urug. [online]. 2016, vol.32, n.3, pp.145-151. ISSN 0303-3295.

Abstract Introduction: In recent years, significant progress has been made in the biological knowledge of acute myeloid leukemia (AML), which has been reflected on treatment of affected patients being guided by cytogenetics and molecular profiling. FLT3 internal tandem duplications (FLT3/ITDs) represent the most frequent mutations in AML and confer a bad prognosis in patients with intermediate cytogenetic risk. It has been reported that the presence of a high FLT3-ITD ratio (relationship between number of ITD carrier allele and wild type allele). Objective: To standardize a technique, still not available in Uruguay, to determine the FLT3-ITD ratio in patients carriers of AML of intermediate cytogenetic risk. To discuss the first cases of AML FLT3+ who underwent ratio analysis. Methods: In order to identify FLT3-ITD, the fragment corresponding to exons 14 and 15 of the gene was amplified in bone marrow samples upon debut of the disease. In the cases it was positive, the FLT3-ITD ratio was determined by the analysis of fragments with capillary electrophoresis. Results: This study presented the standardization of a method to determine the FLT3-ITD ratio and the first cases analysed in our country. Twelve patients were studied and the presence of FLT3-ITD was detected in three of them. In two cases, the FLT3-ITD ratio found was below 0.8 and in one case it was greater than or equal to 0.8. Conclusions: We have a technique to determine the FLT3-ITD ratio with an important prognostic value for patients carriers of AML.

Palabras clave : ACUTE MYELOID LEUKEMIA; FLT3-ITD; CYTOGENETIC ANALYSIS.

        · resumen en Español | Portugués     · texto en Español     · Español ( pdf )

 

Creative Commons License Todo el contenido de esta revista, excepto dónde está identificado, está bajo una Licencia Creative Commons