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Revista Médica del Uruguay

On-line version ISSN 1688-0390


GRILLE, Sofía et al. Estudio de ploidía de células plasmáticas por citometría de flujo en pacientes con mieloma múltiple: primeros casos estudiados en Uruguay. Rev. Méd. Urug. [online]. 2013, vol.29, n.4, pp.226-231. ISSN 1688-0390.

Abstract Introduction: the natural history of multiple myeloma (MM) is heterogeneous, survival rates ranging from a few weeks to over 20 years. Analysis of prognostic factors is essential to decide on a therapy that is adapted to specific risks. Plasma cells ploidy analysis has proved to be a high prognostic value factor. Objective: to standardize a technique, unavailable in Uruguay, consisting in flow cytometry for plasma cells ploidy analysis in order to determine ploidy values in plasma cells. Method: ploidy analysis was performed in the bone marrow, and monoclonal anti-CD38 and CD-138 antibodies were used to mark plasma cells. Propidium iodide was used to study the content of deoxyribonucleic acid (DNA). The DNA was calculated in the analysis (the ratio of the peak mode corresponding to the DNA present in the plasma cells during the Go/G1 phase and the Go/G1 peak mode of residual normal cells). Results: the study presented the standardization of flow cytometry ploidy analysis and the first cases analysed in our country. Nine patients with a diagnosis of MM were studied, having found two hypoploid cases (non-hyperploid), one diploid case (non-hyperploid), and six hyperploid cases. Conclusions: there is a technique for ploidy determination of plasma cells that is simple, fast to perform and has an important prognostic value for patients with MM.


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